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HEK cells were cultured in modified Eagle's medium with 6% fetal bovine serum and antibiotics.
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ified Eagle's medium; FBS, fetal bovine serum; PBS, phosphate-buff- ered saline Joly, C. , Bockaert, J. , Curry, K.
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Eagle's medium, supplemented with KCl (30 mM final), glutamine (2 mM), HEPES Brabet P () PAC1 receptor-deficient mice display impaired insulinotropic.
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